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SKL2001-LCMS-31265-MedChemExpress

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MS Report from Instrument: LCMS-03 File ..a\2018\20180628\20180628\BIZ2018-627-WYQ3.D Tgt Mass (CHM): Injection Date : 28 Jun 18 5:52 pm +0800 Seq. Line : 112 Sample Name : BIZ2018-627-WYQ3 Location : P2-C-03 Acq. Operator : SYSTEM Inj : 1 Spec. Reported : MS Integration Inj Volume : 1 ul Acq. Method : D:\LCMS03\Data\2018\20180628\20180628\1-POS-3MIN.M Analysis Method : D:\LCMS03\Data\2018\20180628\20180628\1-POS-3MIN.M Catalog No : HY-101085 Batch#31265 Method Info : Mobile Phase: A: water(0.01%TFA) B:ACN(0.01%TFA) Gradient: 5% to 95%B within 1.3 min Flow Rate :1.8ml/min Column :SunFire C18, 4.6*50mm,3.5um A-RP-186 Oven Temperature : 45C DAD1 A, Sig=214,4 Ref=off 1.252 mAU 400 200 0 0.277 1.383 1.920 0 0.5 DAD1 B, Sig=254,4 Ref=off mAU 400 200 0 0 0.5 ELS1 A, ELSD Signal mV 50 0 MSD1 TIC, MS File 1500000 1000000 500000 1 1.252 1.5 2 2.5 min 1.362 1.374 1.476 1.724 1.766 1.800 1.973 1 1.325 1.5 2 2.5 min 0.5 1 ES-API, Pos, Scan, Frag: 70 1.281 1.5 2 2.5 min 1.408 1.790 1.877 0 0.5 1 1.5 Ion 287.1, MSD1 287.1,Target Mass 286.1 +H Positive, EIC=286.8:287.8 1000000 500000 0 0 0.5 1 1.5 1.281 2 2.5 min 2 2.5 min Integration Results for DAD1 A, Sig=214,4 Ref=off RetTim Width Area Height Area% MS(+) |-------|--------|--------------|--------------|--------|------| 0.28 0.02 1.40 0.98 0.19 279 1.25 0.02 724.07 676.33 98.08 287 1.38 0.02 3.30 3.16 0.45 505 1.92 0.02 9.44 9.05 1.28 233 Integration Results for DAD1 B, Sig=254,4 Ref=off RetTim Width Area Height Area% MS(+) |-------|--------|--------------|--------------|--------|------| 1.25 0.02 597.87 558.41 98.01 287 1.36 0.01 1.15 1.43 0.19 287 1.37 0.02 3.06 -0.02 0.50 287 1.48 0.02 0.69 0.72 0.11 552 1.72 0.01 2.87 0.16 0.47 233 1.77 0.01 1.85 0.06 0.30 233 1.80 0.01 1.50 0.03 0.25 233 1.97 0.02 1.02 0.08 0.17 233 Integration Results for ELS1 A, ELSD Signal LCMS-03 6/29/2018 9:09:09 AM SYSTEM Page 1 of 4

SKL2001-LCMS-31265-MedChemExpress

MS Report from Instrument: LCMS-03 RetTim Width Area Height Area% MS(+) |-------|--------|--------------|--------------|--------|------| 1.33 0.05 365.12 116.74 100.00 287 Integration Results for MSD1 TIC, MS File RetTim Width Area Height Area% MS(+) |-------|--------|--------------|--------------|--------|------| 1.28 0.06 5302044.50 1446923.88 91.88 287 1.41 0.06 136948.13 36305.43 2.37 505 1.79 0.08 217384.17 45706.32 3.77 233 1.88 0.06 114489.70 30843.61 1.98 289 LCMS-03 6/29/2018 9:09:09 AM SYSTEM Page 2 of 4

SKL2001-LCMS-31265-MedChemExpress

MS Report from Instrument: LCMS-03 Ret. Time: 1.28 ES-API Positive 350000 300000 250000 200000 150000 100000 50000 0 100 200 <<<< POSITIVE SPECTRA >>>> 287.1 288.1 300 400 500 600 700 800 900 m/z Ret. Time: 1.41 ES-API Positive 16000 14000 12000 233.1 102.2 329.1 10000 8000 6000 130.1 163.1 108.1 188.1 247.1 4000 2000 0 100 200 300 400 500 600 700 800 900 m/z 454.1 553.3 556.2 287.1 505.2 414.2 455.2 510.3 477.2 470.1 507.2 527.1 303.1 337.2 392.2 419.1 397.1 448.1 Ret. Time: 1.79 ES-API Positive 16000 14000 12000 10000 8000 6000 217.1 234.1 338.1 188.1 287.0 106.1 314.1 455.1 142.1 163.1 365.3 4000 2000 0 100 200 300 400 500 600 700 800 900 m/z 398.1 420.0 105.1 233.1 278.1 279.1 615.3 521.2 538.2 638.2 816.2 Ret. Time: 1.88 ES-API Positive 233.1 289.1 20000 15000 10000 5000 0 100 200 102.1 308.2 322.2 129.1 234.1 615.2 279.1 338.2 403.2 477.1 638.2 918.2 900 m/z 300 400 500 600 700 800 LCMS-03 6/29/2018 9:09:09 AM SYSTEM Page 3 of 4

SKL2001-LCMS-31265-MedChemExpress

MS Report from Instrument: LCMS-03 *** End of Report *** LCMS-03 6/29/2018 9:09:09 AM SYSTEM Page 4 of 4

SKL2001-LCMS-31265-MedChemExpress

SKL2001-LCMS-31265-MedChemExpress

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