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SKL2001-DataSheet-MedChemExpress

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Inhibitors, Agonists, Screening Libraries www.MedChemExpress.com Data Sheet Product Name: Cat. No.: CAS No.: Molecular Formula: Molecular Weight: Target: Pathway: Solubility: SKL2001 HY-101085 909089-13-0 C14H14N4O 3 286.29 Wnt; β-catenin Stem Cell/Wnt DMSO BIOLOGICAL ACTIVITY:   SKL2001 is an agonist of the Wnt/β-catenin pathway, with anti-cancer activity. IC50 & Target: Wnt/β-catenin[1] In Vitro: SKL2001 is an agonist of the Wnt/β-catenin pathway, and also upregulates the expression of Axin2, a downstream target of the Wnt/β-catenin pathway, but shows no effect on NF-κB, p53 reporter activity and GSK-3β activity. SKL2001 causes osteoblast differentiation (20 and 40 μM) and suppresses preadipocyte differentiation (5, 10, and 30 μM) via the activation of the Wnt/β-catenin pathway. SKL2001 (5, 10, and 30 μM) stabilizes intracellular β-catenin in 3T3-L1 cells[1]. SKL2001 (40 μM) significantly inhibits the HCT116 spheroids. SKL2001 (40 μM) enhances round-shape spheroid formation and E-cadherin expression[2]. proliferation of HCT116 spheroids independently of cytotoxicity and the inhibition is reversible; SKL2001 cuases cell cycle arrest in   Cell Assay: SKL2001 is dissolved in DMSO[1].[1]ST2 cells are cultured on glass chamber slides and then treated with DMSO or SKL2001 PROTOCOL (Extracted from published papers and Only for reference) for 15 h. After treatment, the cells are washed with PBS, fixed with 4% formaldehyde, permeabilized in 0.3% Triton X-100, and blocked in 4% bovine serum albumin for 1 h. The cells are stained with anti-β-catenin antibody and then analyzed by confocal microscopy using a microscope[1].   References: [1]. Gwak J, et al. Small molecule-based disruption of the Axin/β-catenin protein complex regulates mesenchymal stem cell differentiation. ell Res. 2012 Jan; 22(1):237-47. [2]. Ohashi W, et al. SKL2001 suppresses colon cancer spheroid growth through regulation of the E-cadherin/β-Catenin complex. Biochem Biophys Res Commun. 2017 Nov 25;493(3):1342-1348. Caution: Product has not been fully validated for medical applications. For research use only. Tel: 609-228-6898     Fax: 609-228-5909     E-mail: tech@MedChemExpress.com Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA www.MedChemExpress.com

SKL2001-heet-MedChemExpress

SKL2001-heet-MedChemExpress

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